In vivo evaluation of a recombinant N-acylhomoserine lactonase formulated in a hydrogel using a murine model infected with MDR Pseudomonas aeruginosa clinical isolate, CCASUP2

Abstract Failure in the treatment of P. aeruginosa, due to its broad spectrum resistance, has been associated with increased patient mortality. One alternative approach for infection control is quorum quenching which was found decrease virulence such pathogen. In this study, efficiency a recombinant Ahl-1 lactonase formulated as hydrogel investigated multidrug resistant (MDR) aeruginosa infected burn using murine model. The N-acylhomoserine (Ahl-1) hydrogel. To test ability MDR , thermal injury model used. Survival rate, and systemic spread were evaluated. Histopathological examination animal dorsal skin also done monitoring healing cellular changes at site infection. rate treated group 100% relative 40% group. A up 3 logs bacterial count blood samples animals 4 2.3 bacteria lung liver samples, respectively observed. revealed more enhanced process Accordingly, by promoting reducing well decreasing mortality application promising strategy that can be used combat infections.